Culture, Body Fluid (Aerobic and Anaerobic) w Gram Stain
ID: LAB269
Last Review: 09/19/2025

Components
Culture Body Fluid
Gram Stain
 

Specimen Sources
Abdominal Fluid
Amniotic Fluid
Aqueous Fluid
Ascites
Bile
Body Fluid
Bone Marrow
Bursa
Gallbladder
Joint Fluid
Joint, Elbow
Joint, Finger
Joint, Knee
Joint, Left Elbow
Joint, Left Finger
Joint, Left Hip
Joint, Left Knee
Joint, Left Shoulder
Joint, Left Wrist
Joint, Loose Body
Joint, Other
Joint, Right Elbow
Joint, Right Finger
Joint, Right Hip
Joint, Right Knee
Joint, Right Shoulder
Joint, Right Wrist
Joint, Shoulder
Joint, Wrist
Lymphocele
Misc Source
Other
PERICARDIAL FLUID
Pericardium
Peritoneal Fluid
Pleural Fluid
Synovial Fluid
Thoracentesis Fluid
Urine, Kidney
Vitreous Fluid

Turn Around Time (TATs for Specific Labs Below May Differ)
Turn Around Time:
  • ASAP: 9 Day(s)
  • STAT: 9 Day(s)
  • ROUTINE: 9 Day(s)

Performing Labs, Collection Containers and TATs
  DHLN Clinical Laboratory
  Duke Microbiology Laboratory

Clinical Indications
Used to aid in the diagnosis of bacterial infections.

Clinical Significance
In response to infection, fluid may accumulate in any body cavity. Areas of the body from which fluids are commonly sent for microbiological studies include the thorax (thoracentesis or pleural fluid), abdominal cavity (ascites or peritoneal fluid), pericardium (pericardial fluid), synovial fluid, amniotic fluid, and vitreous fluid. Infections in these sites may be caused by aerobes, anaerobes, or a mixture of both. A fluid volume of 1 to 5 mL is adequate for the isolation of most bacteria. Fluid submitted on a swab is suboptimal. False-negative results may occur when insufficient volume is cultured.  False-positive results may occur when sterile technique is not strictly followed.

Interpretation of heavily mixed cultures may be difficult. Guidelines for identification and susceptibility testing of isolates are based on specimen source, method of collection, and numbers of different isolates and specific potential pathogens present.

Methodology
Gram stain, aerobic and anaerobic culture used to aid in the diagnosis of bacterial infections.

Includes identification and susceptibility testing where appropriate at additional charges.

CPT coding cannot be determined before the culture is completed.

Critical Values
No Critical Values