Coronavirus (COVID-19) SARS-CoV-2 PCR Inpatient Only
ID: LAB9985
Last Review: 01/28/2026

Components
Coronavirus (COVID-19) SARS-CoV-2 PCR/NAAT
 

Specimen Sources
BAL
BRONCHIAL LAVAGE
Bronchial Wash
Mini-BAL
Nasal Swab
Nasal Wash
Nasopharyngeal Swab
Rectal
VBAL

Collection Requirements
ACCEPTABLE SPECIMEN:
Nasopharyngeal/Oropharyngeal (NP/OP) swab (preferred), NP swab, OP swab (throat swab), Bronchoalveolar Lavage (BAL), Nasal Swab, Nasopharyngeal Wash, Nasal Wash.
PLACE SWABS IN VTM (Viral Transport Media)

Shipping And Handling
DELIVER IMMEDIATELY TO MICROBIOLOGY IN A TIGHTLY SEALED CONTAINER WITH NO EXTERNAL SPILLAGE.
Refrigerate BAL and endotracheal aspirates. Specimens transported in Viral Transport (VTM) media are stable at ambient temperature.
Causes for Rejection:
1. Specimen container leaking.
2. Specimen not labeled with patient's name, MRN, date and time of collection, and collector's initials.
3. Specimen received on calcium alginate swab.
4. Specimen received on dry swab.
5. Specimen received on swab with wooden shaft.
NOTE: CALCIUM ALGINATE SWABS ARE NOT ACCEPTABLE

Turn Around Time (TATs for Specific Labs Below May Differ)
Turn Around Time:
  • ASAP: 24 Hour(s)
  • STAT: 24 Hour(s)
  • ROUTINE: 24 Hour(s)

Performing Labs, Collection Containers and TATs
  DHLN Clinical Laboratory
  Duke Microbiology Laboratory

Methodology
Real-Time Polymerase Chain Reaction (PCR)

The performance of this PCR test was verified in the Clinical Microbiology Laboratory at Duke University Medical Center.

Additional Information
1.  Nasal swab collection instructions- English:  https://prodduke.sharepoint.com/:b:/s/MicrobiologySharepoint/EYc38gbRN5ROh67CVZdSXsEBfgaObwYIieYdOhWlIGpXhQ?e=SMlpJX
2.  Nasal swab collection instructions- Spanish:  https://prodduke.sharepoint.com/:b:/s/MicrobiologySharepoint/Ed6-oLGUVhxHukAuBtfUoUYBYXM7JYVzU78oopYquc_rUA?e=N45kFj
3.  Nasopharyngeal (NP) swab collection instructions:  https://prodduke.sharepoint.com/:b:/s/MicrobiologySharepoint/EW2-1-nEOo9Kk72SCz6lPWQBUILOcwlzs7XxhgmKKL0EXw?e=hTahuW

Critical Values
No Critical Values